Detailed information of TA system TA161669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 48049..48776
• Replicon: plasmid unnamed1
• Accession: NZ_CP055163
• Organism: Klebsiella pneumoniae strain STEFF_9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUZ51_RS25885
• Protein ID: WP_011251285.1
• Coordinates: 48049..48360 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ51_RS25890
• Protein ID: WP_011251286.1
• Coordinates: 48357..48776 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ51_RS25855 (HUZ51_25805)	43240..44220	+	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-
HUZ51_RS25860 (HUZ51_25810)	44766..44945	-	180	Protein_43	IS5/IS1182 family transposase	-
HUZ51_RS25865 (HUZ51_25815)	44985..46523	-	1539	WP_017901237.1	IS66 family transposase	-
HUZ51_RS25870 (HUZ51_25820)	46572..46919	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HUZ51_RS25875 (HUZ51_25825)	46916..47293	-	378	WP_004118218.1	transposase	-
HUZ51_RS25880 (HUZ51_25830)	47407..47844	+	438	Protein_47	DDE-type integrase/transposase/recombinase	-
HUZ51_RS25885 (HUZ51_25835)	48049..48360	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ51_RS25890 (HUZ51_25840)	48357..48776	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ51_RS25895 (HUZ51_25845)	48846..49259	+	414	WP_017901337.1	hypothetical protein	-
HUZ51_RS25900 (HUZ51_25850)	49305..50273	-	969	WP_074168518.1	IS5-like element IS903B family transposase	-
HUZ51_RS25905 (HUZ51_25855)	50377..50643	+	267	WP_017900868.1	hypothetical protein	-
HUZ51_RS25910 (HUZ51_25860)	50757..51292	-	536	Protein_53	transposase	-
HUZ51_RS25915 (HUZ51_25865)	51817..53184	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(D) / floR / blaDHA-1 / qnrB4 / catB3 / blaOXA-1	-	1..260949	260949
	-	inside	IScluster/Tn	-	-	43240..50885	7645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T161669 WP_011251285.1 NZ_CP055163:48049-48360 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161669 NZ_CP071244:55329-55604 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT161669 WP_011251286.1 NZ_CP055163:48357-48776 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161669 NZ_CP071244:55069-55329 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAACGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1