Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3849587..3850184 Replicon chromosome
Accession NZ_CP055162
Organism Klebsiella pneumoniae strain STEFF_9

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HUZ51_RS18960 Protein ID WP_004142563.1
Coordinates 3849867..3850184 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HUZ51_RS18955 Protein ID WP_004142561.1
Coordinates 3849587..3849874 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ51_RS18925 (3845667) 3845667..3845915 + 249 WP_020325243.1 DUF1158 domain-containing protein -
HUZ51_RS18930 (3845933) 3845933..3846274 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HUZ51_RS18935 (3846305) 3846305..3847420 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
HUZ51_RS18940 (3847600) 3847600..3848181 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
HUZ51_RS18945 (3848181) 3848181..3848549 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
HUZ51_RS18950 (3848669) 3848669..3849322 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HUZ51_RS18955 (3849587) 3849587..3849874 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HUZ51_RS18960 (3849867) 3849867..3850184 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ51_RS18965 (3850369) 3850369..3851412 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
HUZ51_RS18970 (3852082) 3852082..3852948 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HUZ51_RS18975 (3853057) 3853057..3854484 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T161662 WP_004142563.1 NZ_CP055162:c3850184-3849867 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T161662 NZ_CP071242:1603057-1603365 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT161662 WP_004142561.1 NZ_CP055162:c3849874-3849587 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT161662 NZ_CP071242:1602818-1603054 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References