Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3762410..3763209 Replicon chromosome
Accession NZ_CP055113
Organism Shigella flexneri strain SWHIN_101

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag HUZ81_RS18220 Protein ID WP_000347266.1
Coordinates 3762410..3762874 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ81_RS18225 Protein ID WP_001307405.1
Coordinates 3762874..3763209 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ81_RS18190 (3757411) 3757411..3757845 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ81_RS18195 (3757863) 3757863..3758741 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ81_RS18200 (3758731) 3758731..3759510 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ81_RS18205 (3759521) 3759521..3759994 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ81_RS18210 (3760017) 3760017..3761297 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ81_RS18215 (3761546) 3761546..3762355 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ81_RS18220 (3762410) 3762410..3762874 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ81_RS18225 (3762874) 3762874..3763209 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ81_RS18230 (3763358) 3763358..3764929 - 1572 WP_001273741.1 galactarate dehydratase -
HUZ81_RS18235 (3765304) 3765304..3766638 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ81_RS18240 (3766654) 3766654..3767424 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T161598 WP_000347266.1 NZ_CP055113:c3762874-3762410 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161598 NZ_CP071228:c1108036-1107833 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161598 WP_001307405.1 NZ_CP055113:c3763209-3762874 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161598 NZ_CP071228:c1108454-1108086 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References