Detailed information of TA system TA161596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2980058..2980660
• Replicon: chromosome
• Accession: NZ_CP055113
• Organism: Shigella flexneri strain SWHIN_101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HUZ81_RS14415
• Protein ID: WP_001593848.1
• Coordinates: 2980349..2980660 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ81_RS14410
• Protein ID: WP_000356397.1
• Coordinates: 2980058..2980348 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ81_RS14385 (2976003)	2976003..2976905	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUZ81_RS14390 (2976902)	2976902..2977537	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ81_RS14395 (2977534)	2977534..2978463	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ81_RS14400 (2978793)	2978793..2979035	-	243	WP_001087409.1	protein YiiF	-
HUZ81_RS14405 (2979254)	2979254..2979472	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HUZ81_RS14410 (2980058)	2980058..2980348	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ81_RS14415 (2980349)	2980349..2980660	-	312	WP_001593848.1	hypothetical protein	Toxin
HUZ81_RS14420 (2980889)	2980889..2981782	+	894	WP_089616592.1	alpha/beta hydrolase	-
HUZ81_RS14425 (2981861)	2981861..2982802	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUZ81_RS14430 (2982847)	2982847..2983284	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ81_RS14435 (2983281)	2983281..2984153	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ81_RS14440 (2984147)	2984147..2984746	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12174.15 Da
• Isoelectric Point: 9.7791

>T161596 WP_001593848.1 NZ_CP055113:c2980660-2980349 [Shigella flexneri]
MLFIETEIFTEDVQKPLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161596 NZ_CP071226:4534624-4534935 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161596 WP_000356397.1 NZ_CP055113:c2980348-2980058 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161596 NZ_CP071226:4534932-4535354 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG