Detailed information of TA system TA161575

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39708..39972
• Replicon: plasmid unnamed2
• Accession: NZ_CP055111
• Organism: Shigella flexneri strain SWHIN_104

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: A0A8H9BDR8
• Locus tag: HUZ82_RS24395
• Protein ID: WP_023156060.1
• Coordinates: 39820..39972 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	39708..39765 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ82_RS24380 (35750)	35750..36820	-	1071	WP_000151579.1	IncI1-type conjugal transfer protein TrbB	-
HUZ82_RS24385 (36839)	36839..38047	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
HUZ82_RS24390 (38265)	38265..39218	-	954	WP_021513958.1	hypothetical protein	-
- (39412)	39412..39467	-	56	NuclAT_1	-	-
- (39412)	39412..39467	-	56	NuclAT_1	-	-
- (39412)	39412..39467	-	56	NuclAT_1	-	-
- (39412)	39412..39467	-	56	NuclAT_1	-	-
- (39708)	39708..39765	-	58	NuclAT_0	-	Antitoxin
- (39708)	39708..39765	-	58	NuclAT_0	-	Antitoxin
- (39708)	39708..39765	-	58	NuclAT_0	-	Antitoxin
- (39708)	39708..39765	-	58	NuclAT_0	-	Antitoxin
HUZ82_RS24395 (39820)	39820..39972	+	153	WP_023156060.1	Hok/Gef family protein	Toxin
HUZ82_RS24400 (40044)	40044..40295	-	252	WP_021513960.1	hypothetical protein	-
HUZ82_RS24740 (40793)	40793..40888	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
HUZ82_RS24410 (40953)	40953..41129	-	177	WP_001054900.1	hypothetical protein	-
HUZ82_RS24415 (41461)	41461..41670	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
HUZ82_RS24420 (41742)	41742..42404	-	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
HUZ82_RS24425 (42469)	42469..44637	-	2169	WP_000698366.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84883	84883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5809.14 Da
• Isoelectric Point: 8.7948

>T161575 WP_023156060.1 NZ_CP055111:39820-39972 [Shigella flexneri]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGFRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T161575 NZ_CP071222:c3786711-3786322 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT161575 NZ_CP055111:c39765-39708 [Shigella flexneri]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin