Detailed information of TA system TA161566

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 113578..113832
• Replicon: plasmid unnamed1
• Accession: NZ_CP055110
• Organism: Shigella flexneri strain SWHIN_104

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ82_RS23760
• Protein ID: WP_001312851.1
• Coordinates: 113578..113727 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	113771..113832 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ82_RS23720 (109128)	109128..109529	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HUZ82_RS23725 (109462)	109462..109719	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUZ82_RS23730 (109812)	109812..110465	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUZ82_RS23735 (111406)	111406..112263	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HUZ82_RS23740 (112256)	112256..112738	-	483	WP_001273588.1	hypothetical protein	-
HUZ82_RS23745 (112731)	112731..112778	-	48	WP_229471593.1	hypothetical protein	-
HUZ82_RS23750 (112769)	112769..113020	+	252	WP_223195197.1	replication protein RepA	-
HUZ82_RS23755 (113037)	113037..113294	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUZ82_RS23760 (113578)	113578..113727	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (113771)	113771..113832	+	62	NuclAT_1	-	Antitoxin
- (113771)	113771..113832	+	62	NuclAT_1	-	Antitoxin
- (113771)	113771..113832	+	62	NuclAT_1	-	Antitoxin
- (113771)	113771..113832	+	62	NuclAT_1	-	Antitoxin
HUZ82_RS23765 (114088)	114088..114162	-	75	Protein_126	endonuclease	-
HUZ82_RS23770 (114408)	114408..114620	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUZ82_RS23775 (114756)	114756..115316	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUZ82_RS23780 (115419)	115419..116279	-	861	WP_000704514.1	alpha/beta hydrolase	-
HUZ82_RS23785 (116338)	116338..117084	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / dfrA14 / dfrA12 / aadA2 / mph(A) / aph(3')-Ia / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..177080	177080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161566 WP_001312851.1 NZ_CP055110:c113727-113578 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161566 NZ_CP071220:c3786712-3786323 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT161566 NZ_CP055110:113771-113832 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin