Detailed information of TA system TA161565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4589028..4589630
• Replicon: chromosome
• Accession: NZ_CP055109
• Organism: Shigella flexneri strain SWHIN_104

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ82_RS22165
• Protein ID: WP_000897305.1
• Coordinates: 4589319..4589630 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ82_RS22160
• Protein ID: WP_000356397.1
• Coordinates: 4589028..4589318 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ82_RS22135 (4584954)	4584954..4585856	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUZ82_RS22140 (4585853)	4585853..4586488	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ82_RS22145 (4586485)	4586485..4587414	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ82_RS22150 (4587744)	4587744..4587986	-	243	WP_001086388.1	protein YiiF	-
HUZ82_RS22155 (4588205)	4588205..4588423	-	219	WP_001297075.1	CopG family transcriptional regulator	-
HUZ82_RS22160 (4589028)	4589028..4589318	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ82_RS22165 (4589319)	4589319..4589630	-	312	WP_000897305.1	hypothetical protein	Toxin
HUZ82_RS22170 (4589859)	4589859..4590767	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ82_RS22175 (4590831)	4590831..4591772	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HUZ82_RS22180 (4591817)	4591817..4592254	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ82_RS22185 (4592251)	4592251..4593123	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ82_RS22190 (4593117)	4593117..4593716	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161565 WP_000897305.1 NZ_CP055109:c4589630-4589319 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161565 NZ_CP071220:c3109567-3109169 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161565 WP_000356397.1 NZ_CP055109:c4589318-4589028 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161565 NZ_CP071220:c3109800-3109567 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7