Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 594343..595142 Replicon chromosome
Accession NZ_CP055109
Organism Shigella flexneri strain SWHIN_104

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HUZ82_RS02930 Protein ID WP_000347267.1
Coordinates 594343..594807 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ82_RS02935 Protein ID WP_001307405.1
Coordinates 594807..595142 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ82_RS02900 (589344) 589344..589778 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ82_RS02905 (589796) 589796..590674 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ82_RS02910 (590664) 590664..591443 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ82_RS02915 (591454) 591454..591927 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ82_RS02920 (591950) 591950..593230 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ82_RS02925 (593479) 593479..594288 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ82_RS02930 (594343) 594343..594807 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ82_RS02935 (594807) 594807..595142 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ82_RS02940 (595291) 595291..596862 - 1572 WP_001273741.1 galactarate dehydratase -
HUZ82_RS02945 (597237) 597237..598571 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ82_RS02950 (598587) 598587..599357 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T161547 WP_000347267.1 NZ_CP055109:c594807-594343 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161547 NZ_CP071216:c3110824-3110426 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161547 WP_001307405.1 NZ_CP055109:c595142-594807 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161547 NZ_CP071216:c3111057-3110824 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References