Detailed information of TA system TA161507

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108114..108368
• Replicon: plasmid unnamed1
• Accession: NZ_CP055100
• Organism: Shigella flexneri strain SWHIN_107

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ84_RS23925
• Protein ID: WP_001312851.1
• Coordinates: 108114..108263 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	108307..108368 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ84_RS23880 (103664)	103664..104065	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HUZ84_RS23885 (103998)	103998..104255	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUZ84_RS23890 (104348)	104348..105001	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUZ84_RS23895 (105099)	105099..105239	-	141	WP_001333237.1	hypothetical protein	-
HUZ84_RS23900 (105942)	105942..106799	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HUZ84_RS23905 (106792)	106792..107274	-	483	WP_001273588.1	hypothetical protein	-
HUZ84_RS23910 (107267)	107267..107314	-	48	WP_229471593.1	hypothetical protein	-
HUZ84_RS23915 (107305)	107305..107556	+	252	WP_223195197.1	replication protein RepA	-
HUZ84_RS23920 (107573)	107573..107830	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUZ84_RS23925 (108114)	108114..108263	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (108307)	108307..108368	+	62	NuclAT_1	-	Antitoxin
- (108307)	108307..108368	+	62	NuclAT_1	-	Antitoxin
- (108307)	108307..108368	+	62	NuclAT_1	-	Antitoxin
- (108307)	108307..108368	+	62	NuclAT_1	-	Antitoxin
HUZ84_RS23930 (108624)	108624..108698	-	75	Protein_121	endonuclease	-
HUZ84_RS23935 (108944)	108944..109156	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUZ84_RS23940 (109292)	109292..109852	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUZ84_RS23945 (109955)	109955..110815	-	861	WP_000704514.1	alpha/beta hydrolase	-
HUZ84_RS23950 (110874)	110874..111620	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / dfrA14 / dfrA12 / aadA2 / mph(A) / aph(3')-Ia / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..171874	171874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161507 WP_001312851.1 NZ_CP055100:c108263-108114 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161507 NZ_CP071208:55329-55604 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT161507 NZ_CP055100:108307-108368 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin