Detailed information of TA system TA161506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4614012..4614614
• Replicon: chromosome
• Accession: NZ_CP055099
• Organism: Shigella flexneri strain SWHIN_107

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ84_RS22355
• Protein ID: WP_000897305.1
• Coordinates: 4614303..4614614 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ84_RS22350
• Protein ID: WP_000356397.1
• Coordinates: 4614012..4614302 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ84_RS22325 (4609938)	4609938..4610840	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUZ84_RS22330 (4610837)	4610837..4611472	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ84_RS22335 (4611469)	4611469..4612398	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ84_RS22340 (4612728)	4612728..4612970	-	243	WP_001086388.1	protein YiiF	-
HUZ84_RS22345 (4613189)	4613189..4613407	-	219	WP_001297075.1	CopG family transcriptional regulator	-
HUZ84_RS22350 (4614012)	4614012..4614302	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ84_RS22355 (4614303)	4614303..4614614	-	312	WP_000897305.1	hypothetical protein	Toxin
HUZ84_RS22360 (4614843)	4614843..4615751	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ84_RS22365 (4615815)	4615815..4616756	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HUZ84_RS22370 (4616801)	4616801..4617238	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ84_RS22375 (4617235)	4617235..4618107	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ84_RS22380 (4618101)	4618101..4618700	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161506 WP_000897305.1 NZ_CP055099:c4614614-4614303 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161506 NZ_CP071206:4534624-4534935 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161506 WP_000356397.1 NZ_CP055099:c4614302-4614012 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161506 NZ_CP071206:4534932-4535354 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7