Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3749851..3750392 Replicon chromosome
Accession NZ_CP055099
Organism Shigella flexneri strain SWHIN_107

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUZ84_RS18275 Protein ID WP_000615976.1
Coordinates 3750114..3750392 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ84_RS18270 Protein ID WP_000729704.1
Coordinates 3749851..3750111 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ84_RS18245 (3745634) 3745634..3746419 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
HUZ84_RS18250 (3746391) 3746391..3748103 + 1713 Protein_3577 flagellar biosynthesis protein FlhA -
HUZ84_RS18255 (3748208) 3748208..3748486 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUZ84_RS18260 (3748479) 3748479..3748835 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ84_RS18265 (3748892) 3748892..3749665 - 774 WP_000543899.1 C40 family peptidase -
HUZ84_RS18270 (3749851) 3749851..3750111 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ84_RS18275 (3750114) 3750114..3750392 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ84_RS18280 (3750548) 3750548..3751288 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ84_RS18285 (3751259) 3751259..3752026 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ84_RS18290 (3752232) 3752232..3752810 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T161503 WP_000615976.1 NZ_CP055099:3750114-3750392 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161503 NZ_CP071206:c3786732-3786343 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161503 WP_000729704.1 NZ_CP055099:3749851-3750111 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161503 NZ_CP071206:c3786999-3786736 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References