Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3812385..3812982 Replicon chromosome
Accession NZ_CP055096
Organism Klebsiella pneumoniae strain SWHIN_108

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HUZ85_RS18695 Protein ID WP_004142563.1
Coordinates 3812665..3812982 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HUZ85_RS18690 Protein ID WP_004142561.1
Coordinates 3812385..3812672 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ85_RS18660 (3808466) 3808466..3808714 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HUZ85_RS18665 (3808732) 3808732..3809073 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HUZ85_RS18670 (3809104) 3809104..3810219 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HUZ85_RS18675 (3810398) 3810398..3810979 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HUZ85_RS18680 (3810979) 3810979..3811347 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HUZ85_RS18685 (3811467) 3811467..3812120 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HUZ85_RS18690 (3812385) 3812385..3812672 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HUZ85_RS18695 (3812665) 3812665..3812982 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ85_RS18700 (3813167) 3813167..3814210 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
HUZ85_RS18705 (3814877) 3814877..3815743 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
HUZ85_RS18710 (3815852) 3815852..3817279 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T161480 WP_004142563.1 NZ_CP055096:c3812982-3812665 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T161480 NZ_CP055096:c3812982-3812665 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT161480 WP_004142561.1 NZ_CP055096:c3812672-3812385 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT161480 NZ_CP055096:c3812672-3812385 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References