Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3598959..3599500 Replicon chromosome
Accession NZ_CP055092
Organism Shigella flexneri strain SWHIN_110

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag HUZ87_RS17435 Protein ID WP_000615983.1
Coordinates 3599222..3599500 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag HUZ87_RS17430 Protein ID WP_000729703.1
Coordinates 3598959..3599219 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ87_RS17410 (3594634) 3594634..3595419 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HUZ87_RS17415 (3595391) 3595391..3597103 + 1713 Protein_3416 flagellar biosynthesis protein FlhA -
HUZ87_RS17420 (3597327) 3597327..3597824 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HUZ87_RS17425 (3598000) 3598000..3598749 - 750 WP_000056849.1 C40 family peptidase -
HUZ87_RS17430 (3598959) 3598959..3599219 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ87_RS17435 (3599222) 3599222..3599500 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ87_RS17440 (3599656) 3599656..3600396 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ87_RS17445 (3600367) 3600367..3601134 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ87_RS17450 (3601340) 3601340..3601918 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3592762..3608663 15901


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T161461 WP_000615983.1 NZ_CP055092:3599222-3599500 [Shigella flexneri]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161461 NZ_CP071196:4534604-4534915 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT161461 WP_000729703.1 NZ_CP055092:3598959-3599219 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161461 NZ_CP071196:4534912-4535334 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References