Detailed information of TA system TA161439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4870385..4870984
• Replicon: chromosome
• Accession: NZ_CP055089
• Organism: Citrobacter portucalensis strain SWHIN_111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HUZ88_RS23455
• Protein ID: WP_103015341.1
• Coordinates: 4870673..4870984 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ88_RS23450
• Protein ID: WP_038635893.1
• Coordinates: 4870385..4870672 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ88_RS23435 (HUZ88_23380)	4867881..4868783	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
HUZ88_RS23440 (HUZ88_23385)	4868780..4869415	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ88_RS23445 (HUZ88_23390)	4869412..4870341	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HUZ88_RS23450 (HUZ88_23395)	4870385..4870672	-	288	WP_038635893.1	NadS family protein	Antitoxin
HUZ88_RS23455 (HUZ88_23400)	4870673..4870984	-	312	WP_103015341.1	toxin HigB-2	Toxin
HUZ88_RS23460 (HUZ88_23405)	4871205..4872134	+	930	WP_239605720.1	alpha/beta hydrolase	-
HUZ88_RS23465 (HUZ88_23410)	4872219..4872530	+	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	-
HUZ88_RS23470 (HUZ88_23415)	4872527..4872973	+	447	WP_048215203.1	helix-turn-helix domain-containing protein	-
HUZ88_RS23475 (HUZ88_23420)	4872991..4873932	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HUZ88_RS23480 (HUZ88_23425)	4873977..4874414	-	438	WP_003825289.1	D-aminoacyl-tRNA deacylase	-
HUZ88_RS23485 (HUZ88_23430)	4874411..4875283	-	873	WP_008786998.1	virulence factor BrkB family protein	-
HUZ88_RS23490 (HUZ88_23435)	4875277..4875876	-	600	WP_008786997.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12318.48 Da
• Isoelectric Point: 10.4586

>T161439 WP_103015341.1 NZ_CP055089:c4870984-4870673 [Citrobacter portucalensis]
MLFIETEIFTEDVKKLLDDDEYRRLQIFLAIQPDCGDLIQETGGLRKVRWRSRGKGKRSGVRIIYFHQIRKSQIRLLLIY
QKGIKDDLTPQEKVLLRMLNGRW

Nucleotide

• Length: 312 bp

>T161439 NZ_CP071192:c3110845-3110447 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10761.36 Da
• Isoelectric Point: 11.1842

>AT161439 WP_038635893.1 NZ_CP055089:c4870672-4870385 [Citrobacter portucalensis]
MDKALFERLTQSMVQMNEIAEGTRQPSRTFHIDAMKIKEIRQSSGLSQTRFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPKHVIQALR

Nucleotide

• Length: 288 bp

>AT161439 NZ_CP071192:c3111078-3110845 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA