Detailed information of TA system TA161437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4590315..4590979
• Replicon: chromosome
• Accession: NZ_CP055089
• Organism: Citrobacter portucalensis strain SWHIN_111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HUZ88_RS22110
• Protein ID: WP_032942116.1
• Coordinates: 4590677..4590979 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5A9BY49
• Locus tag: HUZ88_RS22105
• Protein ID: WP_008786950.1
• Coordinates: 4590315..4590656 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ88_RS22075 (HUZ88_22035)	4585818..4586576	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
HUZ88_RS22080 (HUZ88_22040)	4586698..4587384	+	687	WP_239605675.1	phosphonate C-P lyase system protein PhnL	-
HUZ88_RS22085 (HUZ88_22045)	4587381..4588517	+	1137	WP_103015272.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HUZ88_RS22090 (HUZ88_22050)	4588520..4589074	+	555	WP_239605676.1	ribose 1,5-bisphosphokinase	-
HUZ88_RS22095 (HUZ88_22055)	4589061..4589495	+	435	WP_239605677.1	aminoalkylphosphonate N-acetyltransferase	-
HUZ88_RS22100 (HUZ88_22060)	4589504..4590262	+	759	WP_239605678.1	phosphonate metabolism protein PhnP	-
HUZ88_RS22105 (HUZ88_22065)	4590315..4590656	-	342	WP_008786950.1	HigA family addiction module antitoxin	Antitoxin
HUZ88_RS22110 (HUZ88_22070)	4590677..4590979	-	303	WP_032942116.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ88_RS22115 (HUZ88_22075)	4591135..4591464	-	330	WP_003031807.1	DDRRRQL repeat protein YjdP	-
HUZ88_RS22120 (HUZ88_22080)	4591534..4593798	-	2265	WP_239605679.1	hybrid sensor histidine kinase/response regulator	-
HUZ88_RS22125 (HUZ88_22085)	4593913..4595436	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11860.49 Da
• Isoelectric Point: 9.9581

>T161437 WP_032942116.1 NZ_CP055089:c4590979-4590677 [Citrobacter portucalensis]
MSKSLNIRNFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWINGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T161437 NZ_CP071192:1601798-1602106 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12986.83 Da
• Isoelectric Point: 6.4743

>AT161437 WP_008786950.1 NZ_CP055089:c4590656-4590315 [Citrobacter portucalensis]
MKQASRKPTTVGDILQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRDVGAKKVA

Nucleotide

• Length: 342 bp

>AT161437 NZ_CP071192:1601559-1601795 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A5A9BY49