Detailed information of TA system TA161425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2392176..2392738
• Replicon: chromosome
• Accession: NZ_CP055089
• Organism: Citrobacter portucalensis strain SWHIN_111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HUZ88_RS11515
• Protein ID: WP_048216313.1
• Coordinates: 2392176..2392454 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: HUZ88_RS11520
• Protein ID: WP_003833018.1
• Coordinates: 2392454..2392738 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ88_RS11495 (HUZ88_11465)	2387254..2387685	-	432	WP_003833007.1	peroxiredoxin OsmC	-
HUZ88_RS11500 (HUZ88_11470)	2387913..2388053	+	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
HUZ88_RS11505 (HUZ88_11475)	2388233..2389930	+	1698	WP_239606479.1	malate dehydrogenase	-
HUZ88_RS11510 (HUZ88_11480)	2390240..2391955	+	1716	WP_239606480.1	ABC-F family ATP-binding cassette domain-containing protein	-
HUZ88_RS11515 (HUZ88_11485)	2392176..2392454	+	279	WP_048216313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ88_RS11520 (HUZ88_11490)	2392454..2392738	+	285	WP_003833018.1	HigA family addiction module antitoxin	Antitoxin
HUZ88_RS11525 (HUZ88_11495)	2392778..2393380	-	603	WP_239606481.1	inorganic diphosphatase	-
HUZ88_RS11530 (HUZ88_11500)	2393510..2394166	-	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
HUZ88_RS11535 (HUZ88_11505)	2394159..2395043	-	885	WP_003833024.1	formate dehydrogenase N subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10681.33 Da
• Isoelectric Point: 9.8965

>T161425 WP_048216313.1 NZ_CP055089:2392176-2392454 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTEINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T161425 NZ_CP071188:4534612-4534923 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT161425 WP_003833018.1 NZ_CP055089:2392454-2392738 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT161425 NZ_CP071188:4534920-4535342 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8