Detailed information of TA system TA161399

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67953..68206
• Replicon: plasmid unnamed2
• Accession: NZ_CP055077
• Organism: Shigella flexneri strain SWHIN_116

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ90_RS23940
• Protein ID: WP_001312851.1
• Coordinates: 68057..68206 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67953..68012 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ90_RS23905 (63729)	63729..64589	+	861	WP_000704523.1	alpha/beta hydrolase	-
HUZ90_RS23910 (64692)	64692..65252	+	561	WP_000139321.1	fertility inhibition protein FinO	-
HUZ90_RS23915 (65381)	65381..65593	+	213	WP_001309245.1	ANR family transcriptional regulator	-
HUZ90_RS23920 (65838)	65838..66299	+	462	WP_001233838.1	thermonuclease family protein	-
HUZ90_RS23925 (66345)	66345..66554	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
HUZ90_RS23930 (66592)	66592..67182	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
HUZ90_RS23935 (67337)	67337..67810	+	474	WP_016240489.1	hypothetical protein	-
- (67953)	67953..68012	-	60	NuclAT_1	-	Antitoxin
- (67953)	67953..68012	-	60	NuclAT_1	-	Antitoxin
- (67953)	67953..68012	-	60	NuclAT_1	-	Antitoxin
- (67953)	67953..68012	-	60	NuclAT_1	-	Antitoxin
HUZ90_RS23940 (68057)	68057..68206	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HUZ90_RS23945 (68491)	68491..68739	+	249	WP_000083839.1	replication regulatory protein RepA	-
HUZ90_RS23950 (68854)	68854..69038	+	185	Protein_89	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..69050	69050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161399 WP_001312851.1 NZ_CP055077:68057-68206 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161399 NZ_CP071172:c2401229-2400954 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT161399 NZ_CP055077:c68012-67953 [Shigella flexneri]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin