Detailed information of TA system TA161396

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26592..26861
• Replicon: plasmid unnamed2
• Accession: NZ_CP055077
• Organism: Shigella flexneri strain SWHIN_116

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ90_RS23690
• Protein ID: WP_001372321.1
• Coordinates: 26736..26861 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26592..26657 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ90_RS23650	21592..22059	-	468	Protein_29	hypothetical protein	-
HUZ90_RS23655	22361..22888	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HUZ90_RS23660	22946..23179	+	234	WP_000006003.1	DUF905 family protein	-
HUZ90_RS23665	23240..25204	+	1965	WP_117389470.1	ParB/RepB/Spo0J family partition protein	-
HUZ90_RS23670	25273..25707	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUZ90_RS23675	25704..26423	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	26435..26659	+	225	NuclAT_0	-	-
-	26435..26659	+	225	NuclAT_0	-	-
-	26435..26659	+	225	NuclAT_0	-	-
-	26435..26659	+	225	NuclAT_0	-	-
HUZ90_RS23680	26444..26623	-	180	WP_001309233.1	hypothetical protein	-
-	26592..26657	+	66	-	-	Antitoxin
HUZ90_RS23685	26645..26794	+	150	Protein_36	plasmid maintenance protein Mok	-
HUZ90_RS23690	26736..26861	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ90_RS23695	27162..27458	-	297	Protein_38	hypothetical protein	-
HUZ90_RS23700	27758..28054	+	297	WP_001272251.1	hypothetical protein	-
HUZ90_RS23705	28165..28986	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HUZ90_RS23710	29283..29885	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HUZ90_RS23715	30206..30589	+	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ90_RS23720	30781..31428	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
HUZ90_RS23725	31548..31775	+	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..69050	69050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T161396 WP_001372321.1 NZ_CP055077:26736-26861 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T161396 NZ_CP071157:962195-962542 [Listeria innocua]
CTGATGGTGAAGCGTGGTGACGTTTACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATAAGGCCTGT
TCTCATCATTCAAAATGATATTGGTAATAGATTCAGTCCAACTGTGATCGTGGCAGCAATAACTGCAAAAATTCAAAAAG
CGAAGTTGCCAACACATGTGGAAGCTACTCGTAAAGACGGCTTTGAAAGAGATTCTGTCATTCTATTAGAACAAATTAGA
ACGATAGATAAACAACGCCTGACAGATAAGATTACACATTTGGACGAGGAACTAATGGCCAAGGTAAATCAGGCACTTGA
AGTCAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT161396 NZ_CP055077:26592-26657 [Shigella flexneri]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin