Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3685145..3685686 Replicon chromosome
Accession NZ_CP055075
Organism Shigella flexneri strain SWHIN_116

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HUZ90_RS18105 Protein ID WP_000615979.1
Coordinates 3685408..3685686 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ90_RS18100 Protein ID WP_000729704.1
Coordinates 3685145..3685405 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ90_RS18080 (3680828) 3680828..3681613 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
HUZ90_RS18085 (3681585) 3681585..3683297 + 1713 Protein_3549 flagellar biosynthesis protein FlhA -
HUZ90_RS18090 (3683513) 3683513..3684010 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
HUZ90_RS18095 (3684186) 3684186..3684959 - 774 WP_133296758.1 C40 family peptidase -
HUZ90_RS18100 (3685145) 3685145..3685405 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ90_RS18105 (3685408) 3685408..3685686 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ90_RS18110 (3685842) 3685842..3686582 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ90_RS18115 (3686553) 3686553..3687320 - 768 WP_000333379.1 class II glutamine amidotransferase -
HUZ90_RS18120 (3687526) 3687526..3688104 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T161388 WP_000615979.1 NZ_CP055075:3685408-3685686 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161388 NZ_CP071150:c3552534-3552316 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161388 WP_000729704.1 NZ_CP055075:3685145-3685405 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161388 NZ_CP071150:c3552935-3552561 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References