Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606228..607027 Replicon chromosome
Accession NZ_CP055075
Organism Shigella flexneri strain SWHIN_116

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HUZ90_RS02950 Protein ID WP_000347251.1
Coordinates 606228..606692 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ90_RS02955 Protein ID WP_001307405.1
Coordinates 606692..607027 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ90_RS02920 (601229) 601229..601663 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ90_RS02925 (601681) 601681..602559 - 879 Protein_574 PTS N-acetylgalactosamine transporter subunit IID -
HUZ90_RS02930 (602549) 602549..603328 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ90_RS02935 (603339) 603339..603812 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ90_RS02940 (603835) 603835..605115 - 1281 Protein_577 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ90_RS02945 (605364) 605364..606173 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ90_RS02950 (606228) 606228..606692 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ90_RS02955 (606692) 606692..607027 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ90_RS02960 (607176) 607176..608747 - 1572 WP_001273753.1 galactarate dehydratase -
HUZ90_RS02965 (609122) 609122..610456 + 1335 WP_074551609.1 galactarate/glucarate/glycerate transporter GarP -
HUZ90_RS02970 (610472) 610472..611242 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T161370 WP_000347251.1 NZ_CP055075:c606692-606228 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161370 NZ_CP071139:c5762000-5761737 [Streptomyces nojiriensis]
GTGAGTGAGTACCGAACCGTCTTCCGCCCCGAGGCGCAGGCCGAACTTCGGAAGATCCCTCGCGACATGGCGTTGCGCAT
CCTGGCCAAGCTGACCGAGCTGGAGACCGACCCTCTCGGCCTCGGCACCACCGCACTCGTGTCCCAGCCCGAACGTCGCC
GCCTGCGCGTCGGCGACTACCGCGTCGTGTACACGATCGACAACGGGGAGCTGGTGGTCTGGGTCGTTCACGTCGGACAC
CGGTCCACCGTCCACGAGACCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161370 WP_001307405.1 NZ_CP055075:c607027-606692 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161370 NZ_CP071139:c5762262-5761993 [Streptomyces nojiriensis]
ATGACGCAGCCACTGCCCATGGAGTCCATCCGCGACGTACGTGCGCACCTGGCGGAGGTCGTGGAGCGCGCGGACCGCGA
CGACGTGCCGACGGTGATCACGCGCCGGGGCAAGGAAGTCGCCGCCATCGTCTCCATCGAGGTACTGCGCAAGTACCAGG
AGTGGGAAGAGCGCGAGATCAACCGGATCATCGACGAGCGCATGGCCAATCCCGCACGCGGCATCCCGATCGAGAACATC
ATGAGGGAGACGCTGGCGCGCGGTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References