Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3761217..3761758 Replicon chromosome
Accession NZ_CP055070
Organism Shigella flexneri strain SWHIN_97

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HUZ91_RS18720 Protein ID WP_000615979.1
Coordinates 3761480..3761758 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ91_RS18715 Protein ID WP_000729704.1
Coordinates 3761217..3761477 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ91_RS18695 (3756900) 3756900..3757685 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
HUZ91_RS18700 (3757657) 3757657..3759369 + 1713 Protein_3673 flagellar biosynthesis protein FlhA -
HUZ91_RS18705 (3759585) 3759585..3760082 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
HUZ91_RS18710 (3760258) 3760258..3761031 - 774 WP_239605907.1 C40 family peptidase -
HUZ91_RS18715 (3761217) 3761217..3761477 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ91_RS18720 (3761480) 3761480..3761758 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ91_RS18725 (3761914) 3761914..3762654 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ91_RS18730 (3762625) 3762625..3763392 - 768 WP_000333379.1 class II glutamine amidotransferase -
HUZ91_RS18735 (3763598) 3763598..3764176 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T161359 WP_000615979.1 NZ_CP055070:3761480-3761758 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161359 NZ_CP071137:c1369757-1369263 [Ferrovum myxofaciens]
ATGAGTCTATCGGCACCACAGTCACTGGATGCGAATCATCGGCTGGAAGAATTTGACTGCGGAAAGCCGGCCCTCACCGA
CTGGCTGCTGCGTCACGCACGCCAAGCTCAAGGTAGCGGTTCAGCGAAAACATTCGTCGCCTGTGATGGAAGTCGCGTTG
CCGGGTACTTCAGTTTGACCGTTGGGCAAATCGACACGCTCGAGGCTCCCGAGCGGATTCGTCGCGGGATGGGCCAGTAC
CCGATTCCGCTGGTCATTCTGGCGCGGCTTGCTGTCGATCTCGATTACCAAAAACAAGGTCTTGGTTTCAGCCTGCTGCA
AGACGCTATTCGCCGAACGGTTGCAATTGCAGAGCATGCAGGTATTCGGGCTCTGCTGACGCACCCTATTGATGCCGAGG
CAGAGACGTTTTATCGGCGATTCGGTTTCGCGTCGACGCCGGTGCGCGAGCAGCAACTGATCCTGCTGCTCAAGGATGCG
CGGCGTTTTGTTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161359 WP_000729704.1 NZ_CP055070:3761217-3761477 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161359 NZ_CP071137:c1370038-1369754 [Ferrovum myxofaciens]
ATGACTGCCACTACTACCGCTCGTTCGGCCCGGCTCGGGCTGCGCGCGACACCTGAACAGGAAACCGTTCTGCGTCGCGC
CGCCGAGGTCGCACACAAATCGTTGACTGATTTCATTCTCGATGCCGCTTACCAAGCGGCCGAGCACACCTTGCTCGATC
AGAGATTGTTTATGGTTTCCGGCAGTCAGTACCAAGCGCTCCTGGAAATGCTGGATCGACCGGAGTCTGATAATCCTGGT
CTGGCCAATTTGTTCTCCCGGCGTCCGGTGTGGGCAGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References