Detailed information of TA system TA161358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3755028..3755722
• Replicon: chromosome
• Accession: NZ_CP055070
• Organism: Shigella flexneri strain SWHIN_97

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HUZ91_RS18680
• Protein ID: WP_001263493.1
• Coordinates: 3755028..3755426 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HUZ91_RS18685
• Protein ID: WP_000554757.1
• Coordinates: 3755429..3755722 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3750688)	3750688..3750768	-	81	NuclAT_11	-	-
- (3750688)	3750688..3750768	-	81	NuclAT_11	-	-
- (3750688)	3750688..3750768	-	81	NuclAT_11	-	-
- (3750688)	3750688..3750768	-	81	NuclAT_11	-	-
HUZ91_RS18650 (3750028)	3750028..3751272	-	1245	WP_000189541.1	esterase FrsA	-
HUZ91_RS18655 (3751364)	3751364..3751822	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HUZ91_RS18660 (3752083)	3752083..3753540	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HUZ91_RS18665 (3753597)	3753597..3754118	-	522	Protein_3666	peptide chain release factor H	-
HUZ91_RS18670 (3754117)	3754117..3754320	-	204	Protein_3667	RtcB family protein	-
HUZ91_RS18675 (3754566)	3754566..3755018	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HUZ91_RS18680 (3755028)	3755028..3755426	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUZ91_RS18685 (3755429)	3755429..3755722	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUZ91_RS18690 (3755774)	3755774..3756829	-	1056	WP_001226164.1	DNA polymerase IV	-
HUZ91_RS18695 (3756900)	3756900..3757685	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
HUZ91_RS18700 (3757657)	3757657..3759369	+	1713	Protein_3673	flagellar biosynthesis protein FlhA	-
HUZ91_RS18705 (3759585)	3759585..3760082	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T161358 WP_001263493.1 NZ_CP055070:c3755426-3755028 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T161358 NZ_CP071137:1162815-1163153 [Ferrovum myxofaciens]
ATGAAAGCCCTGTTTGTCGAGTTGCCCTCCTTTGCCAGATTCAGGGCCGATTACCTGAGTGATGATAGTTTCCGCGCCCT
TCAAAACACTCTGATGAAGAACCCGGAGGCTGGCGACATTATTGAAGGCAGCGGTGGTTTGCGCAAACTGCGTCACGCTG
ATGTCAAACGAGGCAAAGGAAAGCGTGGTGGGTTGCGCGTGATCTACTTCTGGTGGAATGCTGGTCGGCAATTCTGGCTT
TTCACCCTATACGACAAGAATGAAATGGATGACCTGACCATGAAGGGAAAGGGGCTACTCAGGGACATGCTAAAACTTGA
ATTGGAGGAAAGGCGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT161358 WP_000554757.1 NZ_CP055070:c3755722-3755429 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT161358 NZ_CP071137:1163150-1163464 [Ferrovum myxofaciens]
ATGAAAAATCGAAATCTGTTTGCTGAGATTGCCGAGGGTTTCAATGCGTTGGCAGAGGAGCGCGCTGGAAAACGTACGCT
ACGTACTCACGAGGTCGACATGATTCCGACATCAGAGGTTACTGCGGATGAATTGTTGGCTTTGCGGGAAAAGTTGAACC
TATCTCGACCGGTTTTCGCACGCTATCTGCGCACTAACCCGCGTACCCTTGAAAACTGGGAACAGGGTCGCGCCCGTCCC
AATGCCCAGGCTGCCTTGTTGATCCGCCTCGTCGAGCGTTATCCAGATACCATGGAACGTCTCGCAACGGTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6