Detailed information of TA system TA161343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1741448..1741950
• Replicon: chromosome
• Accession: NZ_CP055070
• Organism: Shigella flexneri strain SWHIN_97

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A7I6H4L3
• Locus tag: HUZ91_RS08300
• Protein ID: WP_000767831.1
• Coordinates: 1741696..1741950 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: HUZ91_RS08295
• Protein ID: WP_001259255.1
• Coordinates: 1741448..1741699 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ91_RS08270 (1736625)	1736625..1737692	-	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
HUZ91_RS08275 (1737692)	1737692..1738762	-	1071	WP_000108945.1	histidinol-phosphate transaminase	-
HUZ91_RS08280 (1738759)	1738759..1740063	-	1305	WP_001565639.1	histidinol dehydrogenase	-
HUZ91_RS08285 (1740069)	1740069..1740968	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
HUZ91_RS08290 (1741114)	1741114..1741164	-	51	WP_001364200.1	his operon leader peptide	-
HUZ91_RS08295 (1741448)	1741448..1741699	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HUZ91_RS08300 (1741696)	1741696..1741950	+	255	WP_000767831.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HUZ91_RS08305 (1742033)	1742033..1742857	+	825	WP_000754737.1	SDR family oxidoreductase	-
HUZ91_RS08310 (1742903)	1742903..1743832	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
HUZ91_RS08315 (1744047)	1744047..1744109	+	63	WP_010723108.1	membrane protein YoeI	-
HUZ91_RS08320 (1744099)	1744099..1745457	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
HUZ91_RS08325 (1745636)	1745636..1746694	+	1059	WP_032258476.1	thiosulfate utilization transporter TsuA/YeeE	-
HUZ91_RS08330 (1746708)	1746708..1746935	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10231.67 Da
• Isoelectric Point: 8.8202

>T161343 WP_000767831.1 NZ_CP055070:1741696-1741950 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T161343 NZ_CP071128:c2976905-2976582 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT161343 WP_001259255.1 NZ_CP055070:1741448-1741699 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT161343 NZ_CP071128:c2977230-2976985 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Structures

Toxin

• AlphaFold DB: A0A7I6H4L3

Antitoxin

• AlphaFold DB: A0A7U9LNR0