Detailed information of TA system TA161318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 154052..154779
• Replicon: plasmid unnamed1
• Accession: NZ_CP055062
• Organism: Klebsiella pneumoniae strain SWHE3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUZ66_RS26420
• Protein ID: WP_011251285.1
• Coordinates: 154468..154779 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ66_RS26415
• Protein ID: WP_011251286.1
• Coordinates: 154052..154471 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ66_RS26390 (HUZ66_26310)	149644..151011	+	1368	WP_017900870.1	formimidoylglutamate deiminase	-
HUZ66_RS26395 (HUZ66_26315)	151536..152071	+	536	Protein_166	transposase	-
HUZ66_RS26400 (HUZ66_26320)	152185..152451	-	267	WP_017900868.1	hypothetical protein	-
HUZ66_RS26405 (HUZ66_26325)	152555..153523	+	969	WP_074168518.1	IS5-like element IS903B family transposase	-
HUZ66_RS26410 (HUZ66_26330)	153569..153982	-	414	WP_017901337.1	hypothetical protein	-
HUZ66_RS26415 (HUZ66_26335)	154052..154471	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ66_RS26420 (HUZ66_26340)	154468..154779	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ66_RS26425 (HUZ66_26345)	154984..155421	-	438	Protein_172	DDE-type integrase/transposase/recombinase	-
HUZ66_RS26430 (HUZ66_26350)	155535..155912	+	378	WP_004118218.1	transposase	-
HUZ66_RS26435 (HUZ66_26355)	155909..156256	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HUZ66_RS26440 (HUZ66_26360)	156305..157843	+	1539	WP_017901237.1	IS66 family transposase	-
HUZ66_RS26445 (HUZ66_26365)	157883..158062	+	180	Protein_176	IS5/IS1182 family transposase	-
HUZ66_RS26450 (HUZ66_26370)	158608..159588	-	981	WP_000019441.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB4 / blaDHA-1 / sul1 / blaTEM-1B / aac(3)-IId / qacE / dfrA5 / aph(3'')-Ib / aph(6)-Id / sul2	-	1..263057	263057
	-	inside	IScluster/Tn	-	-	151677..159588	7911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T161318 WP_011251285.1 NZ_CP055062:c154779-154468 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161318 NZ_CP071128:1239606-1239980 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT161318 WP_011251286.1 NZ_CP055062:c154471-154052 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161318 NZ_CP071128:1239412-1239609 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1