Detailed information of TA system TA161289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3844246..3844843
• Replicon: chromosome
• Accession: NZ_CP055057
• Organism: Klebsiella pneumoniae strain SWHE4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: HUZ67_RS18985
• Protein ID: WP_004893639.1
• Coordinates: 3844526..3844843 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: HUZ67_RS18980
• Protein ID: WP_004142561.1
• Coordinates: 3844246..3844533 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ67_RS18950 (HUZ67_18910)	3840326..3840574	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
HUZ67_RS18955 (HUZ67_18915)	3840592..3840933	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HUZ67_RS18960 (HUZ67_18920)	3840964..3842079	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
HUZ67_RS18965 (HUZ67_18925)	3842259..3842843	+	585	WP_002893026.1	TetR/AcrR family transcriptional regulator	-
HUZ67_RS18970 (HUZ67_18930)	3842840..3843208	+	369	WP_002893024.1	MmcQ/YjbR family DNA-binding protein	-
HUZ67_RS18975 (HUZ67_18935)	3843328..3843981	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
HUZ67_RS18980 (HUZ67_18940)	3844246..3844533	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
HUZ67_RS18985 (HUZ67_18945)	3844526..3844843	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ67_RS18990 (HUZ67_18950)	3845028..3846071	-	1044	WP_021314069.1	DUF2157 domain-containing protein	-
HUZ67_RS18995 (HUZ67_18955)	3846737..3847603	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
HUZ67_RS19000 (HUZ67_18960)	3847712..3849139	+	1428	WP_239613042.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T161289 WP_004893639.1 NZ_CP055057:c3844843-3844526 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T161289 NZ_CP071127:c3137004-3136612 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT161289 WP_004142561.1 NZ_CP055057:c3844533-3844246 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT161289 NZ_CP071127:c3137216-3137001 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1