Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 351511..352157 Replicon chromosome
Accession NZ_CP055057
Organism Klebsiella pneumoniae strain SWHE4

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag HUZ67_RS01625 Protein ID WP_016529833.1
Coordinates 351511..351858 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag HUZ67_RS01630 Protein ID WP_002920557.1
Coordinates 351858..352157 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ67_RS01615 (HUZ67_01610) 347437..348870 + 1434 WP_239613178.1 glycogen synthase GlgA -
HUZ67_RS01620 (HUZ67_01615) 348888..351335 + 2448 WP_021312822.1 glycogen phosphorylase -
HUZ67_RS01625 (HUZ67_01620) 351511..351858 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ67_RS01630 (HUZ67_01625) 351858..352157 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
HUZ67_RS01635 (HUZ67_01630) 352220..353728 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
HUZ67_RS01640 (HUZ67_01635) 353933..354262 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
HUZ67_RS01645 (HUZ67_01640) 354313..355143 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
HUZ67_RS01650 (HUZ67_01645) 355193..355951 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T161277 WP_016529833.1 NZ_CP055057:351511-351858 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T161277 NZ_CP071127:c2402506-2402189 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT161277 WP_002920557.1 NZ_CP055057:351858-352157 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT161277 NZ_CP071127:c2402718-2402503 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References