Detailed information of TA system TA161268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5041388..5041990
• Replicon: chromosome
• Accession: NZ_CP055055
• Organism: Shigella dysenteriae strain SWHEFF_49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ68_RS24035
• Protein ID: WP_000897305.1
• Coordinates: 5041388..5041699 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ68_RS24040
• Protein ID: WP_000356397.1
• Coordinates: 5041700..5041990 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ68_RS24015 (5036437)	5036437..5036874	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ68_RS24020 (5036919)	5036919..5037860	+	942	WP_033814966.1	fatty acid biosynthesis protein FabY	-
HUZ68_RS24025 (5038271)	5038271..5039158	+	888	Protein_4706	hypothetical protein	-
HUZ68_RS24030 (5040251)	5040251..5041159	-	909	WP_023155176.1	alpha/beta hydrolase	-
HUZ68_RS24035 (5041388)	5041388..5041699	+	312	WP_000897305.1	hypothetical protein	Toxin
HUZ68_RS24040 (5041700)	5041700..5041990	+	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ68_RS24045 (5042574)	5042574..5042792	+	219	WP_001295676.1	CopG family transcriptional regulator	-
HUZ68_RS24050 (5043009)	5043009..5043251	+	243	WP_001086385.1	protein YiiF	-
HUZ68_RS24055 (5043581)	5043581..5044510	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ68_RS24060 (5044507)	5044507..5045142	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ68_RS24065 (5045139)	5045139..5046041	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161268 WP_000897305.1 NZ_CP055055:5041388-5041699 [Shigella dysenteriae]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161268 NZ_CP071127:c2194969-2194640 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161268 WP_000356397.1 NZ_CP055055:5041700-5041990 [Shigella dysenteriae]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161268 NZ_CP071127:c2195343-2194966 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7