Detailed information of TA system TA161218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4343582..4344309
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: HUZ70_RS20730
• Protein ID: WP_000547555.1
• Coordinates: 4343582..4343893 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ70_RS20735
• Protein ID: WP_000126294.1
• Coordinates: 4343890..4344309 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS20705 (4339517)	4339517..4341226	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
HUZ70_RS20710 (4341236)	4341236..4341778	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HUZ70_RS20715 (4341778)	4341778..4342545	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HUZ70_RS20720 (4342542)	4342542..4342952	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HUZ70_RS20725 (4342945)	4342945..4343415	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HUZ70_RS20730 (4343582)	4343582..4343893	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ70_RS20735 (4343890)	4343890..4344309	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ70_RS20740 (4344388)	4344388..4345812	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
HUZ70_RS20745 (4345821)	4345821..4347278	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HUZ70_RS20750 (4347538)	4347538..4348548	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
HUZ70_RS20755 (4348697)	4348697..4349224	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T161218 WP_000547555.1 NZ_CP055048:4343582-4343893 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161218 NZ_CP071100:c2098526-2098164 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT161218 WP_000126294.1 NZ_CP055048:4343890-4344309 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161218 NZ_CP071100:c2098693-2098523 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: V0YLE2