Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3769850..3770649 Replicon chromosome
Accession NZ_CP055048
Organism Escherichia coli strain SWHEFF_58

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag HUZ70_RS18020 Protein ID WP_000347270.1
Coordinates 3769850..3770314 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag HUZ70_RS18025 Protein ID WP_001551693.1
Coordinates 3770314..3770649 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ70_RS17990 (3764851) 3764851..3765285 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ70_RS17995 (3765303) 3765303..3766181 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ70_RS18000 (3766171) 3766171..3766950 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ70_RS18005 (3766961) 3766961..3767434 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ70_RS18010 (3767457) 3767457..3768737 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ70_RS18015 (3768986) 3768986..3769795 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ70_RS18020 (3769850) 3769850..3770314 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ70_RS18025 (3770314) 3770314..3770649 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ70_RS18030 (3770798) 3770798..3772369 - 1572 WP_069903725.1 galactarate dehydratase -
HUZ70_RS18035 (3772744) 3772744..3774078 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HUZ70_RS18040 (3774094) 3774094..3774864 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T161214 WP_000347270.1 NZ_CP055048:c3770314-3769850 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T161214 NZ_CP071100:c836071-835883 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT161214 WP_001551693.1 NZ_CP055048:c3770649-3770314 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161214 NZ_CP071100:c836360-836100 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References