Detailed information of TA system TA161212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2938002..2938604
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ70_RS14050
• Protein ID: WP_000897305.1
• Coordinates: 2938293..2938604 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ70_RS14045
• Protein ID: WP_000356395.1
• Coordinates: 2938002..2938292 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS14010 (2933626)	2933626..2934528	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUZ70_RS14015 (2934525)	2934525..2935160	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ70_RS14020 (2935157)	2935157..2936086	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HUZ70_RS14025 (2936268)	2936268..2936510	-	243	WP_001309881.1	CopG family transcriptional regulator	-
HUZ70_RS14030 (2936729)	2936729..2936947	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HUZ70_RS14035 (2937366)	2937366..2937644	-	279	WP_001315112.1	hypothetical protein	-
HUZ70_RS14040 (2937696)	2937696..2937917	-	222	WP_001550354.1	hypothetical protein	-
HUZ70_RS14045 (2938002)	2938002..2938292	-	291	WP_000356395.1	NadS family protein	Antitoxin
HUZ70_RS14050 (2938293)	2938293..2938604	-	312	WP_000897305.1	hypothetical protein	Toxin
HUZ70_RS14055 (2938833)	2938833..2939741	+	909	WP_001550353.1	alpha/beta hydrolase	-
HUZ70_RS14060 (2939909)	2939909..2940823	-	915	WP_109553727.1	transposase	-
HUZ70_RS14065 (2940836)	2940836..2941723	-	888	Protein_2749	hypothetical protein	-
HUZ70_RS14070 (2942139)	2942139..2943080	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUZ70_RS14075 (2943125)	2943125..2943562	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161212 WP_000897305.1 NZ_CP055048:c2938604-2938293 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161212 NZ_CP071098:667217-667471 [Fusobacterium animalis]
ATGTTATTGACTTGGACTGATTTTGCTTGGGAACAATATGAAGAATTACAAGAGAAAGATAAAAGACTTGTAAAAAAGAT
AAATACACTTATTAAGGATATCAAAAGAAATGGTAATGAAGGAATAGGAAAACCAGAACCTTTACAACATGAACTAAGTG
GTTATTGGAGTAGAAGAATTGATGATAAAAATAGATTAGTTTATAAAATATCAGATACTCAAATAACAATAGTTGCTTGT
GCAAATCATTATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT161212 WP_000356395.1 NZ_CP055048:c2938292-2938002 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161212 NZ_CP071098:666960-667217 [Fusobacterium animalis]
ATGATAGCAACAAATTATTCAGAGGTTAGAAATAATTTAAAAACTTATTGTGATAAGGCAACAAAGGACTATGAAACTAT
AATTATTACAAGAAAAAATAATGAAAATGTTGTTTTAATGAGTGAAGAAGAATATAACAATCTTATGGAAAACCTTTATA
TAAGATCAAATCTTAAATATTATCAAAGACTTGTAGAAAGTATAAAAGAAGCTGAAAAGGGTAATGTTAAAGAACATGAT
TTAATAGAGGTGGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7