Detailed information of TA system TA161209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2050382..2051076
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: HUZ70_RS09915
• Protein ID: WP_001263491.1
• Coordinates: 2050382..2050780 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: HUZ70_RS09920
• Protein ID: WP_000554755.1
• Coordinates: 2050783..2051076 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (2046211)	2046211..2046291	-	81	NuclAT_10	-	-
- (2046211)	2046211..2046291	-	81	NuclAT_10	-	-
- (2046211)	2046211..2046291	-	81	NuclAT_10	-	-
- (2046211)	2046211..2046291	-	81	NuclAT_10	-	-
HUZ70_RS09885 (2045551)	2045551..2046795	-	1245	WP_000189541.1	esterase FrsA	-
HUZ70_RS09890 (2046887)	2046887..2047345	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HUZ70_RS09895 (2047606)	2047606..2049063	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
HUZ70_RS09900 (2049120)	2049120..2049472	-	353	Protein_1942	peptide chain release factor H	-
HUZ70_RS09905 (2049468)	2049468..2049674	-	207	Protein_1943	RtcB family protein	-
HUZ70_RS09910 (2049920)	2049920..2050372	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
HUZ70_RS09915 (2050382)	2050382..2050780	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUZ70_RS09920 (2050783)	2050783..2051076	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUZ70_RS09925 (2051128)	2051128..2052183	-	1056	WP_001550655.1	DNA polymerase IV	-
HUZ70_RS09930 (2052254)	2052254..2053039	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
HUZ70_RS09935 (2053011)	2053011..2054723	+	1713	Protein_1949	flagellar biosynthesis protein FlhA	-
HUZ70_RS09940 (2054828)	2054828..2055106	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
HUZ70_RS09945 (2055099)	2055099..2055455	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2040318..2051076	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T161209 WP_001263491.1 NZ_CP055048:c2050780-2050382 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T161209 NZ_CP071097:49805-50074 [Fusobacterium animalis]
ATGACCTATACAGTAAATACAACACCACAATTTGATAAAGAATTTAAGAAATTAGATAAGTTTACTCAAAAAATCTTGGC
CACTTGGATTGATAAAAATCTTGAAGGTTGTGAAAATCCACGTATACAAGGAAAAGGATTAACTTCTAATCGCAGCGGAC
AGTGGAGGTATAGAATAGGAGATTATAGATTAATTTGTATAATAGAAGATAAAGAACTTATTATTTTAGCTCTTACAGTT
GGACATAGAAGAGATGTTTATGAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT161209 WP_000554755.1 NZ_CP055048:c2051076-2050783 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT161209 NZ_CP071097:49587-49808 [Fusobacterium animalis]
ATGGCATTTTCAATAAGACTTACAGAAGAAGAAAAAAAATTAGCAACTAGTTATGCAAAATTGAACTCTTTATCTTTAGG
AGAAGCTTTTAAAAAAGCATTATTTGAAAAAATAGAAGAAGAATATGATATTGTTGTTGCTGAAGAGTCCTTTAAAAAAT
ATATTGATAGTGGAAAGAAAAGTCGTCCATTTTCTGAATTAAAAAAGGAATTAGGATTATGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5