Detailed information of TA system TA161170

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2134176..2134547
• Replicon: chromosome
• Accession: NZ_CP055041
• Organism: Shigella flexneri strain SWHEFF_59

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HUZ71_RS10350
• Protein ID: WP_001317028.1
• Coordinates: 2134353..2134547 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2134176..2134354 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ71_RS10320 (2129354)	2129354..2130727	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
HUZ71_RS10325 (2130822)	2130822..2131519	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
HUZ71_RS10330 (2131633)	2131633..2132568	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HUZ71_RS10335 (2132620)	2132620..2133855	-	1236	WP_000040852.1	site-specific integrase	-
HUZ71_RS10340 (2133857)	2133857..2134072	-	216	WP_000079604.1	excisionase XisR	-
- (2134176)	2134176..2134354	+	179	NuclAT_0	-	Antitoxin
- (2134176)	2134176..2134354	+	179	NuclAT_0	-	Antitoxin
- (2134176)	2134176..2134354	+	179	NuclAT_0	-	Antitoxin
- (2134176)	2134176..2134354	+	179	NuclAT_0	-	Antitoxin
HUZ71_RS10345 (2134151)	2134151..2134360	-	210	WP_072644107.1	double-strand break reduction protein RcbA	-
HUZ71_RS10350 (2134353)	2134353..2134547	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HUZ71_RS10355 (2134604)	2134604..2135413	-	810	WP_000166319.1	recombination protein RecT	-
HUZ71_RS10360 (2135406)	2135406..2138006	-	2601	WP_072644104.1	exodeoxyribonuclease VIII	-
HUZ71_RS10365 (2138108)	2138108..2138383	-	276	WP_000632297.1	protein RacC	-
HUZ71_RS10370 (2138458)	2138458..2138628	-	171	WP_001352098.1	YdaE family protein	-
HUZ71_RS10375 (2138628)	2138628..2138849	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2095443..2164736	69293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T161170 WP_001317028.1 NZ_CP055041:c2134547-2134353 [Shigella flexneri]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T161170 NZ_CP071073:c4193093-4192797 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT161170 NZ_CP055041:2134176-2134354 [Shigella flexneri]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
TAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin