Detailed information of TA system TA161168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1010552..1011246
• Replicon: chromosome
• Accession: NZ_CP055041
• Organism: Shigella flexneri strain SWHEFF_59

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HUZ71_RS04810
• Protein ID: WP_001263493.1
• Coordinates: 1010848..1011246 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: HUZ71_RS04805
• Protein ID: WP_072644207.1
• Coordinates: 1010552..1010845 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ71_RS04785 (1006184)	1006184..1006681	+	498	WP_000006257.1	REP-associated tyrosine transposase RayT	-
HUZ71_RS04790 (1006905)	1006905..1008617	-	1713	Protein_922	flagellar biosynthesis protein FlhA	-
HUZ71_RS04795 (1008589)	1008589..1009374	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HUZ71_RS04800 (1009445)	1009445..1010500	+	1056	WP_001226164.1	DNA polymerase IV	-
HUZ71_RS04805 (1010552)	1010552..1010845	+	294	WP_072644207.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUZ71_RS04810 (1010848)	1010848..1011246	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUZ71_RS04815 (1011256)	1011256..1011708	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HUZ71_RS04820 (1011954)	1011954..1012157	+	204	Protein_928	RtcB family protein	-
HUZ71_RS04825 (1012156)	1012156..1012677	+	522	Protein_929	peptide chain release factor H	-
HUZ71_RS04830 (1012734)	1012734..1014191	-	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
HUZ71_RS04835 (1014452)	1014452..1014910	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (1015506)	1015506..1015586	+	81	NuclAT_11	-	-
- (1015506)	1015506..1015586	+	81	NuclAT_11	-	-
- (1015506)	1015506..1015586	+	81	NuclAT_11	-	-
- (1015506)	1015506..1015586	+	81	NuclAT_11	-	-
HUZ71_RS04840 (1015002)	1015002..1016246	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	hcp1/tssD1 / tssA / gmhA/lpcA / fdeC	975050..1066888	91838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T161168 WP_001263493.1 NZ_CP055041:1010848-1011246 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T161168 NZ_CP071073:c2791879-2791776 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11249.85 Da
• Isoelectric Point: 5.9050

>AT161168 WP_072644207.1 NZ_CP055041:1010552-1010845 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT161168 NZ_CP071073:2791738-2792009 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1EXB8