Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3781688..3782229 Replicon chromosome
Accession NZ_CP055037
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HUZ72_RS18440 Protein ID WP_000615979.1
Coordinates 3781951..3782229 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ72_RS18435 Protein ID WP_000729704.1
Coordinates 3781688..3781948 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS18415 (3777371) 3777371..3778156 - 786 WP_222683313.1 putative lateral flagellar export/assembly protein LafU -
HUZ72_RS18420 (3778128) 3778128..3779840 + 1713 Protein_3620 flagellar biosynthesis protein FlhA -
HUZ72_RS18425 (3780056) 3780056..3780553 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
HUZ72_RS18430 (3780729) 3780729..3781502 - 774 WP_001353765.1 C40 family peptidase -
HUZ72_RS18435 (3781688) 3781688..3781948 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ72_RS18440 (3781951) 3781951..3782229 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ72_RS18445 (3782385) 3782385..3783125 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ72_RS18450 (3783096) 3783096..3783863 - 768 WP_000333379.1 class II glutamine amidotransferase -
HUZ72_RS18455 (3784069) 3784069..3784647 - 579 WP_047656750.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T161158 WP_000615979.1 NZ_CP055037:3781951-3782229 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161158 NZ_CP071073:c677059-676907 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161158 WP_000729704.1 NZ_CP055037:3781688-3781948 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161158 NZ_CP071073:677108-677165 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References