Detailed information of TA system TA161157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3775499..3776193
• Replicon: chromosome
• Accession: NZ_CP055037
• Organism: Shigella flexneri strain SWHEFF_60

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HUZ72_RS18400
• Protein ID: WP_001263493.1
• Coordinates: 3775499..3775897 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HUZ72_RS18405
• Protein ID: WP_000554757.1
• Coordinates: 3775900..3776193 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3771159)	3771159..3771239	-	81	NuclAT_11	-	-
- (3771159)	3771159..3771239	-	81	NuclAT_11	-	-
- (3771159)	3771159..3771239	-	81	NuclAT_11	-	-
- (3771159)	3771159..3771239	-	81	NuclAT_11	-	-
HUZ72_RS18370 (3770499)	3770499..3771743	-	1245	WP_000189541.1	esterase FrsA	-
HUZ72_RS18375 (3771835)	3771835..3772293	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HUZ72_RS18380 (3772554)	3772554..3774011	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HUZ72_RS18385 (3774068)	3774068..3774589	-	522	Protein_3613	peptide chain release factor H	-
HUZ72_RS18390 (3774588)	3774588..3774791	-	204	Protein_3614	RtcB family protein	-
HUZ72_RS18395 (3775037)	3775037..3775489	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HUZ72_RS18400 (3775499)	3775499..3775897	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUZ72_RS18405 (3775900)	3775900..3776193	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUZ72_RS18410 (3776245)	3776245..3777300	-	1056	WP_001226164.1	DNA polymerase IV	-
HUZ72_RS18415 (3777371)	3777371..3778156	-	786	WP_222683313.1	putative lateral flagellar export/assembly protein LafU	-
HUZ72_RS18420 (3778128)	3778128..3779840	+	1713	Protein_3620	flagellar biosynthesis protein FlhA	-
HUZ72_RS18425 (3780056)	3780056..3780553	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T161157 WP_001263493.1 NZ_CP055037:c3775897-3775499 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T161157 NZ_CP071073:c583952-583611 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT161157 WP_000554757.1 NZ_CP055037:c3776193-3775900 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT161157 NZ_CP071073:583947-584023 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6