Detailed information of TA system TA161143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1876406..1876908
• Replicon: chromosome
• Accession: NZ_CP055037
• Organism: Shigella flexneri strain SWHEFF_60

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: HUZ72_RS08800
• Protein ID: WP_000767829.1
• Coordinates: 1876654..1876908 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: HUZ72_RS08795
• Protein ID: WP_001259255.1
• Coordinates: 1876406..1876657 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ72_RS08770 (1871583)	1871583..1872650	-	1068	WP_000080090.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
HUZ72_RS08775 (1872650)	1872650..1873720	-	1071	WP_053274541.1	histidinol-phosphate transaminase	-
HUZ72_RS08780 (1873717)	1873717..1875021	-	1305	WP_001446927.1	histidinol dehydrogenase	-
HUZ72_RS08785 (1875027)	1875027..1875926	-	900	WP_099098318.1	ATP phosphoribosyltransferase	-
HUZ72_RS08790 (1876072)	1876072..1876122	-	51	WP_001364200.1	his operon leader peptide	-
HUZ72_RS08795 (1876406)	1876406..1876657	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HUZ72_RS08800 (1876654)	1876654..1876908	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HUZ72_RS08805 (1876991)	1876991..1877815	+	825	WP_000754737.1	SDR family oxidoreductase	-
HUZ72_RS08810 (1877861)	1877861..1878790	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
HUZ72_RS08815 (1879005)	1879005..1879067	+	63	WP_010723108.1	membrane protein YoeI	-
HUZ72_RS08820 (1879057)	1879057..1880415	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
HUZ72_RS08825 (1880594)	1880594..1881652	+	1059	WP_000492330.1	thiosulfate utilization transporter TsuA/YeeE	-
HUZ72_RS08830 (1881666)	1881666..1881893	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T161143 WP_000767829.1 NZ_CP055037:1876654-1876908 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T161143 NZ_CP071063:c4577671-4577486 [Enterobacter mori]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCGGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCACCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCTAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGGCAGGCAGGAATTAAACACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT161143 WP_001259255.1 NZ_CP055037:1876406-1876657 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT161143 NZ_CP071063:c4577471-4577064 [Enterobacter mori]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGTTTTTTCCCTGACGTTCCAGGCTGCTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCACGTGATGCGCTCAGCGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGATGCACTGCCTCTTCCGGGTAATGTGGAAGCGCATCTGGAAAGTCGTCCTGAGGATTTCACCGGCGGGCAATGGCTG
TTGGTGGATATTAATATGAAACAGTTCGACGGCAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
GATTGATTCCTATGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CTGGGTAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0