Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 656180..656907 Replicon chromosome
Accession NZ_CP055037
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUZ72_RS03205 Protein ID WP_000550189.1
Coordinates 656180..656494 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUZ72_RS03210 Protein ID WP_000560255.1
Coordinates 656491..656907 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS03185 (652346) 652346..653332 - 987 WP_053274491.1 Gfo/Idh/MocA family oxidoreductase -
HUZ72_RS03190 (653411) 653411..654094 - 684 WP_001183041.1 vancomycin high temperature exclusion protein -
HUZ72_RS03195 (654171) 654171..654674 - 504 WP_001295542.1 M48 family metallopeptidase -
HUZ72_RS03200 (654759) 654759..655895 + 1137 WP_000018690.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUZ72_RS03205 (656180) 656180..656494 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUZ72_RS03210 (656491) 656491..656907 + 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUZ72_RS03215 (656952) 656952..658970 - 2019 WP_239637458.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUZ72_RS03220 (659296) 659296..661647 - 2352 WP_239637459.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T161138 WP_000550189.1 NZ_CP055037:656180-656494 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T161138 NZ_CP071063:c1067439-1067221 [Enterobacter mori]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCTTGCGACGTTGTCAGTCAATAGACACGCTCGAACGTGT
CATCGAAAAAAATAAATACGAGCTGTCTGATAATGAACTGGCTGTATTTTATTCAGCCGCTGACCATCGGCTCGCTGAGC
TGACCATGAATAAGCTGTATGACAAAATCCCAACATCCGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT161138 WP_000560255.1 NZ_CP055037:656491-656907 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT161138 NZ_CP071063:c1067841-1067467 [Enterobacter mori]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTGAATGACCCAACGTCTGCAATCAATTTACAGTTGAACGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGACGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGCTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGCTTCGTCAACGTGAGCAGAGCAAACCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References