Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606669..607468 Replicon chromosome
Accession NZ_CP055037
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HUZ72_RS02950 Protein ID WP_000347251.1
Coordinates 606669..607133 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ72_RS02955 Protein ID WP_001307405.1
Coordinates 607133..607468 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS02920 (601670) 601670..602104 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ72_RS02925 (602122) 602122..603000 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ72_RS02930 (602990) 602990..603769 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ72_RS02935 (603780) 603780..604253 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ72_RS02940 (604276) 604276..605556 - 1281 WP_239637455.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ72_RS02945 (605805) 605805..606614 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ72_RS02950 (606669) 606669..607133 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ72_RS02955 (607133) 607133..607468 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ72_RS02960 (607617) 607617..609188 - 1572 WP_001273753.1 galactarate dehydratase -
HUZ72_RS02965 (609563) 609563..610897 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ72_RS02970 (610913) 610913..611683 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T161137 WP_000347251.1 NZ_CP055037:c607133-606669 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161137 NZ_CP071063:535373-535693 [Enterobacter mori]
ATGCACATTTCATCTGTACCGGTCACGGTGCCGGTTTCGTCACGCCTGTCGCCCGTACAGGTCTGGCAGCAATTGTTAAC
GTATCTGCTGGAACACCATTACGGCCTGACTCTCAACGACACGCCATTCCACGACGATACGGCCATTGAGGAGCATATCG
ACGCGGGAGTCACGCTTGCCGATGCTGTTAATTTCCTGGTGGAACGTTATGAACTGGTCCGCATCGACCGTAAAGGATTT
ACCTGGCAGGAGCAGACGCCATTTCTGACCGCGACTGATATTCTCAGAGCCAGGCGAGCTACCGGATTAATAAATACCTG
A

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161137 WP_001307405.1 NZ_CP055037:c607468-607133 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161137 NZ_CP071063:535018-535341 [Enterobacter mori]
ATGCAAAATTCATGGGGACTGAAGCGTGACATCTCGCCGTGCTTCGGTGCAAGGCTTGTGCAAGAAGGCTGTCGCCTGCA
TTTTCTGGCAGACCGTGCAGGTATTAGCGGTGCGTTTAACGAGGAGGAGGCGCTGCGTCTGGATCAGGCATTTCCGCTAG
TGATGAAACAGCTTGAATTGATGCTGACAACCGGTGAACTGAATCCGCGCCATCAGCATTGCGTCACGTTGTATCATAAC
GGACTAACTTGTGAAGCTGACACTCTCGGCTCTCATGGCTATGTTTACATCGTTATTTATCCTCAGGCATCTGCAACCAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References