Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3705448..3705989 Replicon chromosome
Accession NZ_CP055030
Organism Shigella flexneri strain SWHEFF_64

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUZ74_RS18035 Protein ID WP_000615976.1
Coordinates 3705711..3705989 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ74_RS18030 Protein ID WP_000729704.1
Coordinates 3705448..3705708 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ74_RS18010 (3701123) 3701123..3701908 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
HUZ74_RS18015 (3701880) 3701880..3703592 + 1713 Protein_3542 flagellar biosynthesis protein FlhA -
HUZ74_RS18020 (3703816) 3703816..3704313 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HUZ74_RS18025 (3704489) 3704489..3705262 - 774 WP_015675129.1 C40 family peptidase -
HUZ74_RS18030 (3705448) 3705448..3705708 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ74_RS18035 (3705711) 3705711..3705989 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ74_RS18040 (3706145) 3706145..3706885 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ74_RS18045 (3706856) 3706856..3707623 - 768 WP_000333379.1 class II glutamine amidotransferase -
HUZ74_RS18050 (3707829) 3707829..3708407 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3698289..3721506 23217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T161113 WP_000615976.1 NZ_CP055030:3705711-3705989 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161113 NZ_CP071028:c154874-154431 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161113 WP_000729704.1 NZ_CP055030:3705448-3705708 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161113 NZ_CP071028:c155101-154871 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References