Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prlF-yhaV (relBE)/YhaV-PrlF |
| Location | 606083..606882 | Replicon | chromosome |
| Accession | NZ_CP055030 | ||
| Organism | Shigella flexneri strain SWHEFF_64 | ||
Toxin (Protein)
| Gene name | yhaV | Uniprot ID | V0SSH7 |
| Locus tag | HUZ74_RS02925 | Protein ID | WP_000347273.1 |
| Coordinates | 606083..606547 (-) | Length | 155 a.a. |
Antitoxin (Protein)
| Gene name | prlF | Uniprot ID | S1EB98 |
| Locus tag | HUZ74_RS02930 | Protein ID | WP_001307405.1 |
| Coordinates | 606547..606882 (-) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ74_RS02895 (601084) | 601084..601518 | - | 435 | WP_000948824.1 | PTS galactosamine/N-acetylgalactosamine transporter subunit IIA | - |
| HUZ74_RS02900 (601536) | 601536..602414 | - | 879 | WP_001300474.1 | PTS N-acetylgalactosamine transporter subunit IID | - |
| HUZ74_RS02905 (602404) | 602404..603183 | - | 780 | WP_000406214.1 | PTS N-acetylgalactosamine transporter subunit IIC | - |
| HUZ74_RS02910 (603194) | 603194..603667 | - | 474 | WP_001295547.1 | PTS N-acetylgalactosamine transporter subunit IIB | - |
| HUZ74_RS02915 (603690) | 603690..604970 | - | 1281 | WP_000681903.1 | tagatose-bisphosphate aldolase subunit KbaZ | - |
| HUZ74_RS02920 (605219) | 605219..606028 | + | 810 | WP_000072187.1 | aga operon transcriptional regulator AgaR | - |
| HUZ74_RS02925 (606083) | 606083..606547 | - | 465 | WP_000347273.1 | type II toxin-antitoxin system ribonuclease toxin YhaV | Toxin |
| HUZ74_RS02930 (606547) | 606547..606882 | - | 336 | WP_001307405.1 | type II toxin-antitoxin system antitoxin PrlF | Antitoxin |
| HUZ74_RS02935 (607031) | 607031..608602 | - | 1572 | WP_001723936.1 | galactarate dehydratase | - |
| HUZ74_RS02940 (608977) | 608977..610311 | + | 1335 | WP_001723935.1 | galactarate/glucarate/glycerate transporter GarP | - |
| HUZ74_RS02945 (610327) | 610327..611097 | + | 771 | WP_001723934.1 | 2-dehydro-3-deoxyglucarate aldolase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 606083..617755 | 11672 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 155 a.a. Molecular weight: 17836.25 Da Isoelectric Point: 9.6924
>T161093 WP_000347273.1 NZ_CP055030:c606547-606083 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH
Download Length: 465 bp
>T161093 NZ_CP071025:c2277421-2276936 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG
Antitoxin
Download Length: 112 a.a. Molecular weight: 12358.94 Da Isoelectric Point: 4.8616
>AT161093 WP_001307405.1 NZ_CP055030:c606882-606547 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
Download Length: 336 bp
>AT161093 NZ_CP071025:c2277675-2277409 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0SSH7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVC7 |