Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 627179..627906 Replicon chromosome
Accession NZ_CP055024
Organism Shigella flexneri strain SWHEFF_67

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUZ75_RS03095 Protein ID WP_000550189.1
Coordinates 627179..627493 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUZ75_RS03100 Protein ID WP_000560270.1
Coordinates 627490..627906 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ75_RS03075 (623337) 623337..624323 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HUZ75_RS03080 (624402) 624402..625094 - 693 Protein_604 vancomycin high temperature exclusion protein -
HUZ75_RS03085 (625171) 625171..625674 - 504 WP_001333820.1 M48 family metallopeptidase -
HUZ75_RS03090 (625759) 625759..626895 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUZ75_RS03095 (627179) 627179..627493 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUZ75_RS03100 (627490) 627490..627906 + 417 WP_000560270.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUZ75_RS03105 (627951) 627951..629969 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUZ75_RS03110 (630395) 630395..632746 - 2352 WP_072712653.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T161073 WP_000550189.1 NZ_CP055024:627179-627493 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T161073 NZ_CP071021:30480-30794 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15021.45 Da        Isoelectric Point: 4.3430

>AT161073 WP_000560270.1 NZ_CP055024:627490-627906 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDYLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT161073 NZ_CP071021:30791-31210 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References