Detailed information of TA system TA161068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3958601..3959295
• Replicon: chromosome
• Accession: NZ_CP055022
• Organism: Escherichia coli strain SWHEFF_68

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: HUZ76_RS18970
• Protein ID: WP_239635631.1
• Coordinates: 3958601..3958999 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: HUZ76_RS18975
• Protein ID: WP_000554755.1
• Coordinates: 3959002..3959295 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ76_RS18940 (3953863)	3953863..3955320	+	1458	WP_022645227.1	cytosol nonspecific dipeptidase	-
HUZ76_RS18945 (3955329)	3955329..3955610	+	282	WP_022645226.1	hypothetical protein	-
HUZ76_RS18950 (3955627)	3955627..3956136	-	510	WP_001361775.1	metal-dependent hydrolase	-
HUZ76_RS18955 (3956198)	3956198..3956812	-	615	WP_022645225.1	peptide chain release factor H	-
HUZ76_RS18960 (3956809)	3956809..3957948	-	1140	WP_022645224.1	RNA ligase RtcB family protein	-
HUZ76_RS18965 (3958139)	3958139..3958591	-	453	WP_023909048.1	GNAT family N-acetyltransferase	-
HUZ76_RS18970 (3958601)	3958601..3958999	-	399	WP_239635631.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUZ76_RS18975 (3959002)	3959002..3959295	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUZ76_RS18980 (3959347)	3959347..3960402	-	1056	WP_022645222.1	DNA polymerase IV	-
HUZ76_RS18985 (3960473)	3960473..3961396	-	924	WP_001232555.1	putative lateral flagellar export/assembly protein LafU	-
HUZ76_RS18990 (3961399)	3961399..3962262	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
HUZ76_RS18995 (3962275)	3962275..3962991	-	717	WP_088129960.1	FliA/WhiG family RNA polymerase sigma factor	-
HUZ76_RS19000 (3963011)	3963011..3963478	-	468	WP_000725260.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15441.90 Da
• Isoelectric Point: 8.9511

>T161068 WP_239635631.1 NZ_CP055022:c3958999-3958601 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTAVFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T161068 NZ_CP071019:3454409-3454789 [Enterobacter hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT161068 WP_000554755.1 NZ_CP055022:c3959295-3959002 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT161068 NZ_CP071019:3454050-3454376 [Enterobacter hormaechei]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829G9H5