Detailed information of TA system TA161048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 631920..632719
• Replicon: chromosome
• Accession: NZ_CP055022
• Organism: Escherichia coli strain SWHEFF_68

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HUZ76_RS03010
• Protein ID: WP_000347273.1
• Coordinates: 631920..632384 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HUZ76_RS03015
• Protein ID: WP_001308975.1
• Coordinates: 632384..632719 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ76_RS02980 (626921)	626921..627355	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HUZ76_RS02985 (627373)	627373..628251	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HUZ76_RS02990 (628241)	628241..629020	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HUZ76_RS02995 (629031)	629031..629504	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HUZ76_RS03000 (629527)	629527..630807	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HUZ76_RS03005 (631056)	631056..631865	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HUZ76_RS03010 (631920)	631920..632384	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HUZ76_RS03015 (632384)	632384..632719	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HUZ76_RS03020 (632868)	632868..634439	-	1572	WP_001273756.1	galactarate dehydratase	-
HUZ76_RS03025 (634814)	634814..636148	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HUZ76_RS03030 (636164)	636164..636934	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T161048 WP_000347273.1 NZ_CP055022:c632384-631920 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T161048 NZ_CP071015:c2279972-2279487 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT161048 WP_001308975.1 NZ_CP055022:c632719-632384 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT161048 NZ_CP071015:c2280226-2279960 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08