Detailed information of TA system TA161041

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 99184..99438
• Replicon: plasmid unnamed1
• Accession: NZ_CP055020
• Organism: Escherichia coli strain SWHEFF_69

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ77_RS24125
• Protein ID: WP_001312851.1
• Coordinates: 99289..99438 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	99184..99245 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ77_RS24090 (94836)	94836..95048	+	213	WP_005012601.1	hypothetical protein	-
HUZ77_RS24095 (95349)	95349..95438	-	90	Protein_118	IS1 family transposase	-
HUZ77_RS24100 (95493)	95493..96170	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
HUZ77_RS24105 (96170)	96170..96517	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HUZ77_RS24110 (96537)	96537..98108	+	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
HUZ77_RS24115 (98146)	98146..98762	-	617	Protein_122	IS1-like element IS1A family transposase	-
HUZ77_RS24120 (98863)	98863..99045	+	183	WP_000968309.1	hypothetical protein	-
- (99184)	99184..99245	-	62	NuclAT_2	-	Antitoxin
- (99184)	99184..99245	-	62	NuclAT_2	-	Antitoxin
- (99184)	99184..99245	-	62	NuclAT_2	-	Antitoxin
- (99184)	99184..99245	-	62	NuclAT_2	-	Antitoxin
HUZ77_RS24125 (99289)	99289..99438	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HUZ77_RS24130 (99722)	99722..99979	+	258	WP_000083833.1	replication regulatory protein RepA	-
HUZ77_RS24135 (100215)	100215..100289	+	75	WP_032336874.1	RepA leader peptide Tap	-
HUZ77_RS24140 (100282)	100282..100728	+	447	Protein_127	plasmid replication initiator RepA	-
HUZ77_RS24145 (100728)	100728..101342	-	615	Protein_128	VENN motif pre-toxin domain-containing protein	-
HUZ77_RS24150 (102049)	102049..103269	+	1221	WP_000410951.1	arginine deiminase	-
HUZ77_RS24155 (103280)	103280..104191	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / aac(3)-IId / aph(3')-Ia / sul3 / blaTEM-1B / sitABCD	iutA / iucD / iucC / iucB / iucA	1..154739	154739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161041 WP_001312851.1 NZ_CP055020:99289-99438 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161041 NZ_CP071013:3454410-3454790 [Enterobacter hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT161041 NZ_CP055020:c99245-99184 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin