Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2403440..2404002 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HUZ77_RS11350 Protein ID WP_000605676.1
Coordinates 2403724..2404002 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HUZ77_RS11345 Protein ID WP_000781370.1
Coordinates 2403440..2403724 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS11330 (2398799) 2398799..2401846 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
HUZ77_RS11335 (2401859) 2401859..2402743 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
HUZ77_RS11340 (2402736) 2402736..2403389 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
HUZ77_RS11345 (2403440) 2403440..2403724 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HUZ77_RS11350 (2403724) 2403724..2404002 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ77_RS11355 (2404188) 2404188..2405198 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HUZ77_RS11360 (2405332) 2405332..2407029 - 1698 WP_000433464.1 malate dehydrogenase -
HUZ77_RS11365 (2407186) 2407186..2407323 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HUZ77_RS11370 (2407425) 2407425..2407640 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HUZ77_RS11375 (2407985) 2407985..2408416 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T161020 WP_000605676.1 NZ_CP055019:c2404002-2403724 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T161020 NZ_CP071009:c1080529-1080311 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT161020 WP_000781370.1 NZ_CP055019:c2403724-2403440 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT161020 NZ_CP071009:c1080932-1080558 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References