Detailed information of TA system TA161012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 678939..679738
• Replicon: chromosome
• Accession: NZ_CP055019
• Organism: Escherichia coli strain SWHEFF_69

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HUZ77_RS03305
• Protein ID: WP_000347273.1
• Coordinates: 678939..679403 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HUZ77_RS03310
• Protein ID: WP_001308975.1
• Coordinates: 679403..679738 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ77_RS03275 (673940)	673940..674374	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HUZ77_RS03280 (674392)	674392..675270	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HUZ77_RS03285 (675260)	675260..676039	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HUZ77_RS03290 (676050)	676050..676523	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HUZ77_RS03295 (676546)	676546..677826	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HUZ77_RS03300 (678075)	678075..678884	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HUZ77_RS03305 (678939)	678939..679403	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HUZ77_RS03310 (679403)	679403..679738	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HUZ77_RS03315 (679887)	679887..681458	-	1572	WP_001273756.1	galactarate dehydratase	-
HUZ77_RS03320 (681833)	681833..683167	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HUZ77_RS03325 (683183)	683183..683953	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T161012 WP_000347273.1 NZ_CP055019:c679403-678939 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T161012 NZ_CP070997:c2978946-2978659 [Acinetobacter indicus]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTTGAAACTGCAAG
CCTTATATTTGAAGATCCTCTACGGATCTCAATCCAAGACAGACATACCAATGGGGAAGAGCGTTGGCAAACCATTGGTA
AGGTCAAAGGAGTACTGATGTTATTAGTTGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATAAGAATTATTAGT
GCAAGACAGGTAACAAAAGCGGAGCGAGATAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT161012 WP_001308975.1 NZ_CP055019:c679738-679403 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT161012 NZ_CP070997:c2978672-2978358 [Acinetobacter indicus]
ATGAGCATGGTTAGACACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAACAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATGCCTGAAATTACAGAATGGAGCAATGCTGTACGCT
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGTTATCAGACACGTATGAATGAAATCTTGCGGGATGCCATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08