Detailed information of TA system TA161005

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 159337..159763
• Replicon: plasmid unnamed1
• Accession: NZ_CP055016
• Organism: Shigella flexneri strain SWHEFF_71

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ78_RS25280
• Protein ID: WP_001372321.1
• Coordinates: 159337..159462 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	159539..159763 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ78_RS25240 (154708)	154708..155397	-	690	WP_000283379.1	conjugal transfer transcriptional regulator TraJ	-
HUZ78_RS25245 (155584)	155584..155967	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ78_RS25250 (156300)	156300..156890	+	591	WP_222908884.1	transglycosylase SLT domain-containing protein	-
HUZ78_RS25255 (157187)	157187..158008	-	822	WP_001241365.1	DUF932 domain-containing protein	-
HUZ78_RS25260 (158128)	158128..158415	-	288	WP_000107535.1	hypothetical protein	-
HUZ78_RS25265 (158440)	158440..158646	-	207	WP_033804333.1	hypothetical protein	-
HUZ78_RS25270 (158716)	158716..158889	+	174	Protein_176	hypothetical protein	-
HUZ78_RS25275 (158887)	158887..159117	-	231	WP_071845785.1	hypothetical protein	-
HUZ78_RS25280 (159337)	159337..159462	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ78_RS25285 (159404)	159404..159553	-	150	Protein_179	plasmid maintenance protein Mok	-
- (159539)	159539..159763	-	225	NuclAT_0	-	Antitoxin
- (159539)	159539..159763	-	225	NuclAT_0	-	Antitoxin
- (159539)	159539..159763	-	225	NuclAT_0	-	Antitoxin
- (159539)	159539..159763	-	225	NuclAT_0	-	Antitoxin
HUZ78_RS25290 (159575)	159575..159763	+	189	WP_001299721.1	hypothetical protein	-
HUZ78_RS25295 (159732)	159732..160494	-	763	Protein_181	plasmid SOS inhibition protein A	-
HUZ78_RS25300 (160491)	160491..160925	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
HUZ78_RS25305 (160980)	160980..162938	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
HUZ78_RS25310 (162997)	162997..163230	-	234	WP_000006004.1	DUF905 domain-containing protein	-
HUZ78_RS25315 (163286)	163286..163813	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
HUZ78_RS25320 (164286)	164286..164552	-	267	WP_072254135.1	hypothetical protein	-
HUZ78_RS25325 (164456)	164456..164704	-	249	WP_012311402.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD	exeG / exeE / stcE	1..183928	183928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T161005 WP_001372321.1 NZ_CP055016:c159462-159337 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T161005 NZ_CP070991:2688818-2689180 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT161005 NZ_CP055016:c159763-159539 [Shigella flexneri]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin