Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3921547..3922088 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUZ78_RS19250 Protein ID WP_000615976.1
Coordinates 3921547..3921825 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ78_RS19255 Protein ID WP_000729704.1
Coordinates 3921828..3922088 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS19235 (3919129) 3919129..3919707 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HUZ78_RS19240 (3919913) 3919913..3920680 + 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ78_RS19245 (3920651) 3920651..3921391 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ78_RS19250 (3921547) 3921547..3921825 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ78_RS19255 (3921828) 3921828..3922088 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ78_RS19260 (3922274) 3922274..3923047 + 774 WP_000543897.1 C40 family peptidase -
HUZ78_RS19265 (3923104) 3923104..3923460 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ78_RS19270 (3923453) 3923453..3923731 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUZ78_RS19275 (3923836) 3923836..3925548 - 1713 Protein_3761 flagellar biosynthesis protein FlhA -
HUZ78_RS19280 (3925520) 3925520..3926305 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T161000 WP_000615976.1 NZ_CP055015:c3921825-3921547 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161000 NZ_CP070991:c1638843-1638538 [Staphylococcus aureus]
ATGAATTTGGAAATTAAAGATTTGTTTAGCGATTTGAAATTGTTGAAAGATAGATTCGAAGATTTAAAGGATAATCATGG
TTGGCATTTTGAGGAGCTATATCCACATGAACCAAATCATGTCTTAAATAAAGATGAATTAATTGGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTTATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161000 WP_000729704.1 NZ_CP055015:c3922088-3921828 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161000 NZ_CP070991:c1639055-1638846 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGGTTAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGGAAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References