Detailed information of TA system TA160997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3079278..3079880
• Replicon: chromosome
• Accession: NZ_CP055015
• Organism: Shigella flexneri strain SWHEFF_71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ78_RS15400
• Protein ID: WP_000897305.1
• Coordinates: 3079278..3079589 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ78_RS15405
• Protein ID: WP_000356397.1
• Coordinates: 3079590..3079880 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ78_RS15375 (3075192)	3075192..3075791	+	600	WP_001295269.1	glucose-1-phosphatase	-
HUZ78_RS15380 (3075785)	3075785..3076657	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ78_RS15385 (3076654)	3076654..3077091	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ78_RS15390 (3077136)	3077136..3078077	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HUZ78_RS15395 (3078141)	3078141..3079049	-	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ78_RS15400 (3079278)	3079278..3079589	+	312	WP_000897305.1	hypothetical protein	Toxin
HUZ78_RS15405 (3079590)	3079590..3079880	+	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ78_RS15410 (3080485)	3080485..3080703	+	219	WP_001295676.1	CopG family transcriptional regulator	-
HUZ78_RS15415 (3080922)	3080922..3081164	+	243	WP_001086388.1	protein YiiF	-
HUZ78_RS15420 (3081393)	3081393..3082373	-	981	WP_000399679.1	IS110-like element IS621 family transposase	-
HUZ78_RS15425 (3082772)	3082772..3083701	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ78_RS15430 (3083698)	3083698..3084333	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3081393..3082373	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T160997 WP_000897305.1 NZ_CP055015:3079278-3079589 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T160997 NZ_CP070991:173490-173642 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGGACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAGGACAGTCAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT160997 WP_000356397.1 NZ_CP055015:3079590-3079880 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT160997 NZ_CP070991:172866-173465 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTTATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7