Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2302968..2303767 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HUZ78_RS11620 Protein ID WP_000347267.1
Coordinates 2303303..2303767 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ78_RS11615 Protein ID WP_001307405.1
Coordinates 2302968..2303303 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS11600 (2298753) 2298753..2299523 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
HUZ78_RS11605 (2299539) 2299539..2300873 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ78_RS11610 (2301248) 2301248..2302819 + 1572 WP_001273741.1 galactarate dehydratase -
HUZ78_RS11615 (2302968) 2302968..2303303 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ78_RS11620 (2303303) 2303303..2303767 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ78_RS11625 (2303822) 2303822..2304631 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ78_RS11630 (2304880) 2304880..2306160 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ78_RS11635 (2306183) 2306183..2306656 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ78_RS11640 (2306667) 2306667..2307446 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ78_RS11645 (2307436) 2307436..2308314 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ78_RS11650 (2308332) 2308332..2308766 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T160995 WP_000347267.1 NZ_CP055015:2303303-2303767 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T160995 NZ_CP070986:c2494252-2493986 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT160995 WP_001307405.1 NZ_CP055015:2302968-2303303 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT160995 NZ_CP070986:c2494509-2494252 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References